Are histochemical methods for estrogen receptor valid?
نویسندگان
چکیده
منابع مشابه
Biochemical assessment of histochemical methods for oestrogen receptor localisation.
The biochemical validity of published labelled-ligand and ligand-antibody methods for the histological localisation of oestrogen receptors (ER) in breast carcinomas was assessed by measuring specific and non-specific binding of 3H-oestradiol to pretreated MCF-7 monolayers; the monolayers had been either frozen and thawed, then air-dried or acetone-fixed, or fixed in formaldehyde to reproduce th...
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امروزه استفاده از منابع انرژی پراکنده کاربرد وسیعی یافته است . اگر چه این منابع بسیاری از مشکلات شبکه را حل می کنند اما زیاد شدن آنها مسائل فراوانی برای سیستم قدرت به همراه دارد . استفاده از میکروشبکه راه حلی است که علاوه بر استفاده از مزایای منابع انرژی پراکنده برخی از مشکلات ایجاد شده توسط آنها را نیز منتفی می کند . همچنین میکروشبکه ها کیفیت برق و قابلیت اطمینان تامین انرژی مشترکان را افزایش ...
15 صفحه اولComparison of histochemical and biochemical assays for estrogen receptor in human breast cancer cell lines.
Two human breast cancer lines, MCF-7 and T47D cells, were investigated for the presence of estrogen receptor (ER) by biochemical and histochemical techniques. Using the dextran-coated charcoal technique and isoelectric focusing, MCF-7 cells were ER positive, and T47D cells were ER negative. Fluorescein conjugates to 17 beta-estradiol by the sixth carbon (17 beta-estradiol-6-carboxymethyloxime:b...
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To investigate the development of induced cervicovaginal carcinoma in mice several experiments were carried out. In 1 ex periment 3 groups of 10 C3H mice received 0.5 /zg of estradiol17/8-monobenzoate s.c. twice weekly. Two groups of mice had been ovariectomized. One of these groups had been painted on the eetocervix with chloroform. Mice of the other group received on the eetocervix 20-methylc...
متن کاملRat Uterine Oxytocin Receptor and Estrogen Receptor α and β mRNA Levels are Regulated by Estrogen Through Multiple Estrogen Receptors
Estrogen action is mediated through several types of receptors (ERs), such as ERα, ERβ and putative membrane ERs. Oxytocin receptor (OTR) and ER expression levels in the rat uterus are regulated by estrogen; however, which types of ERs are involved has not been elucidated. This study examined OTR, ERα and ERβ levels in ovariectomized rats treated with 17β-estradiol (E2), an ERα agonist (PPT), a...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1980
ISSN: 0022-1554,1551-5044
DOI: 10.1177/28.8.7003005